Stock

FESA Number

7923

Strain Name

StarD10-tg-F3

Mutation Strain Type

Mutation Type Mutation Subtype Strain Type
IMSR - Transgenic IMSR - mutant strain

Attribution

Institution name Depositor / Originator Name
Imperial College Originator Retained
Imperial College Depositor Retained

Gene/Allele Information

Allele Name Allele MGI ID Gene Name Gene MGI ID Chromosome
Stard10 MGI:1860093 7

Phenotypic Description

Overexpression/Repression of the transgene when crossed with mice expressing (reverse) tetracycline transactivator and given doxycycline. The transgene copy number for this strain was evaluated by qPCR and estimated to be 15 copies ([14.50 - 16.44], n = 4). The expression level in islets level was measured by western blot after induction by 2g/L doxycycline in drinking water and estimated to be 250 % of the control expression. In vivo phenotype: Crossing with RIP7-rtTA mice allowed overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line showed no differences with control mice on chow diet. On high fat diet (65% calories from fat; from 5-week-old) the transgenic mice had a lower weight and better glucose tolerance than control at 16-week-old without significant difference in the intra-peritoneal insulin tolerance test.

Publications

EMMA ID

EM:09782

Genetic Status

GMO

Background Strain Name

C57BL/6J

Background Strain MGI ID

MGI:3028467

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