Mutation Strain Type
|Mutation Type||Mutation Subtype||Strain Type|
|IMSR - Transgenic||IMSR - mutant strain|
|Institution name||Depositor / Originator||Name|
|Allele Name||Allele MGI ID||Gene Name||Gene MGI ID||Chromosome|
Overexpression/Repression of the transgene when crossed with mice expressing (reverse) tetracycline transactivator and given doxycycline. The transgene copy number for this strain was evaluated by qPCR and estimated to be 15 copies ([14.50 - 16.44], n = 4). The expression level in islets level was measured by western blot after induction by 2g/L doxycycline in drinking water and estimated to be 250 % of the control expression. In vivo phenotype: Crossing with RIP7-rtTA mice allowed overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line showed no differences with control mice on chow diet. On high fat diet (65% calories from fat; from 5-week-old) the transgenic mice had a lower weight and better glucose tolerance than control at 16-week-old without significant difference in the intra-peritoneal insulin tolerance test.