Mutation Strain Type
|Mutation Type||Mutation Subtype||Strain Type|
|IMSR - Transgenic||IMSR - mutant strain|
|Institution name||Depositor / Originator||Name|
|Allele Name||Allele MGI ID||Gene Name||Gene MGI ID||Chromosome|
Overexpression of the transgene when crossed with mouse expressing the reverse tetracycline transactivator and given doxycycline (possibility of a tissue specific overexpression). The transgene copy number for this line was evaluated by qPCR and estimated at 25 copies ([23.88 - 25.43], n = 14). The expression level in islets level was measured by western blot after induction by 2g/L doxycycline in drinking water and estimated to be 120% of the control expression. In vivo phenotype: The animals were crossed with RIP7-rtTA mice allowing overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line had no differences with control mice on chow diet. This line has not yet been tested on HFD.