Stock

FESA Number

7924

Strain Name

StarD10-tg-F10

Mutation Strain Type

Mutation Type Mutation Subtype Strain Type
IMSR - Transgenic IMSR - mutant strain

Attribution

Institution name Depositor / Originator Name
Imperial College Originator Retained
Imperial College Depositor Retained

Gene/Allele Information

Allele Name Allele MGI ID Gene Name Gene MGI ID Chromosome
Stard10 MGI:1860093 7

Phenotypic Description

Overexpression of the transgene when crossed with mouse expressing the reverse tetracycline transactivator and given doxycycline (possibility of a tissue specific overexpression). The transgene copy number for this line was evaluated by qPCR and estimated at 25 copies ([23.88 - 25.43], n = 14). The expression level in islets level was measured by western blot after induction by 2g/L doxycycline in drinking water and estimated to be 120% of the control expression. In vivo phenotype: The animals were crossed with RIP7-rtTA mice allowing overexpression of the transgene specifically in pancreatic beta-cells. The doxycycline was added in drinking water (2g/L) from 5 weeks of age and intra-peritoneal glucose tolerance tests (IPGTT, 1g/kg glucose) were performed at 8, 12 and 16-week-old. The resulting line had no differences with control mice on chow diet. This line has not yet been tested on HFD.

Publications

EMMA ID

EM:09784

Genetic Status

GMO

Background Strain Name

C57BL/6J

Background Strain MGI ID

MGI:3028467

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