| Black coat colour. The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). The non-agouti locus from C57BL/6J has been bred to congenicity on a 129S9/SvEvH background. This stock is homozygous for non-agouti (a/a). When chimaeric mice derived from 129S9/SvEvH ES cells are tested for germ line transmission it is often performed against a C57BL/6J background to use the non-agouti phenotype as a marker of non-GLT. Mice with Agouti coats are derived from the ES cells and therefore potential carriers of the genetic alteration. These mice however are F1’s between C57BL/6 and 129S9/SvEvH. By transferring the a locus to the 129S9/SvEvH background we be able to maintain the 129 genetic background whilst checking for GLT.
This strain allows chimaeric mice derived from 129S9/SvEvH ES cells to be tested for germline transmission using coat colour but ensuring that the genetic background is maintained as 129S9/SvEvH.
Barbaric I et al.( 2007). A new coat color mouse line for testing germline transmission of embryonic stem cells while retaining an inbred genetic background. J. Am. Assoc. Lab. Anim. Sci. 46(3):37-40. |
